Synthesis, Spectral Characteristics of Cyanine Dye Cy3 Bonding Cdte/cds Core/shell Structure Quantum Dots

Using mercaptoethylamine as a stabilizing agent, CdTe/CdS core/shell structure quantum dots (QDs) were prepared by a one-pot synthesis based on a simple high pressure autoclave. In addition, a new compound QDs-Cy3 was synthesized by chemical bonding between QDs and Cy3, which bond each other in a complex embrace involving aminogroup on QDs and carboxyl on Cy3. The products structures were characterized by Infrared spectra and Differential thermal analysis, and the spectral characteristics were revealed. The fluorescence spectra results indicated that the fluorescence intensity and peak shape of QDs-Cy3, which was compared with cyanine dye Cy3, had an evident change. The fluorescence spectra also indicated QDs and Cy3 may occur to fluorescence resonance energy transfer (FRET) by bonding between QDs and Cy3. Bovine serum albumin were labeled by QDs-Cy3 and results showed that the fluorescence intensity were higher than that of unlabelled. The FRET characteristics between QDs and Cy3 are being investigated.